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1. Compensation in flowjo how to#
2. Compensation in flowjo software#
3. Compensation in flowjo professional#

When making multiple matrices like this, you only need to repeat the drags of the successive Cy5PE populations followed by steps 4 and 5, saving a different matrix for each set of reagents. You will need to collect a singly-stained compensation control sample for each different Cy5PE reagent, and then construct a different matrix for each of these. From this menu, you will be able to select a scaling type. Flow Jo should pick the positive & negative populations automatically. Click the compensation icon and select the parameters you are using. Different Cy5PE reagents (even from the same manufacturer) have different spectra and thus require different compensations. How do I change the scale of my data In FlowJo If post acquisition compensation has been applied to the file, display transformations in FlowJo can be enabled using the T-button and selecting Customize Axis. To use the spectral compensation feature, you’ll need FlowJo version 10.6 or later (FCS express now has this feature also) Drag in the single stained controls. This is true, for instance, when you are using different lots of tandem dyes (like Cy5PE). North American Track is a distributor of high-quality aftermarket undercarriage parts and a full-service undercarriage track shop.We work on all earthmoving equipment, including Caterpillar ®, John Deere and more.With dozens of quality aftermarket brands in stock, our knowledgable staff will take the operating conditions of each customers machine. In some cases, you will need multiple compensation matrices for the same experiment. You can follow this example on your own computer by downloading the Compensation Demo Data.

Compensation in flowjo software#

Drag each of the positive and negative populations from the workspace window into the appropriate box in the compensation dialog window.

Compensation in flowjo professional#

Bring up the Compensation Definition dialog window, or Compensation Wizard. This brief guide is to help users new to compensation calculations and experienced flow-maestros alike breeze through this process in a painless and professional fashion. Set a gate for the positive and negative populations for each singly-stained sample. Set a gate for the positive and negative populations for each singly-stained sample.But, FlowJo can't be used to fix overcompensated fcs 2.0 data, since the uncompensated data is lost. Similarly, if your data is undercompensated, it can be fixed.

If you acquire uncompensated data on a Calibur, FlowJo can compensate and analyze it.

Compensation in flowjo how to#

This is a simple example showing how to do three-color compensation in FlowJo. FlowJo can do post-processing compensation of either fcs 3.0 digital or fcs 2.0 analog data.